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Flow cytometric technique for quantitating cytotoxic response to photodynamic therapy
- Source :
- Photochemistry and photobiology. 63(1)
- Publication Year :
- 1996
-
Abstract
- A simple flow cytometric technique for rapid measurement of multilog cytotoxic responses to photosensitization of cellular systems is described. This technique is particularly useful for cell lines with a low colony-forming efficiency, for which a nonclonogenic assay is required. The assay separates cell-sized objects from cellular debris by gating on forward scatter versus side scatter, identifies viable cells by positive calcein AM and negative ethidium homodimer-1 staining and measures cell concentration relative to an internal standard of polystyrene beads. Large numbers of cells can be analyzed rapidly. Two patient-derived small cell lung cancer cell lines, NCI-H209 and SV-E, were used to test the technique. Photordiation survival curves of the response of these cell lines to 5-aminolevulinic acid-induced protoprophyrin IX photosensitization correlated with the extent of photosensitizer accumulation. There was good agreement between the results obtained using the tritiated thymidine incorporation assay and the flow cytometric cytotoxicity assay. The technique can be used to measure cytotoxic responses to photosensitization of cell lines regardless of their plating efficiencies.
- Subjects :
- Lung Neoplasms
medicine.diagnostic_test
Cell Survival
medicine.medical_treatment
Protoporphyrins
Photodynamic therapy
General Medicine
Aminolevulinic Acid
Biology
Flow Cytometry
Biochemistry
Molecular biology
Flow cytometry
Staining
Photochemotherapy
Cell culture
medicine
Tumor Cells, Cultured
Cytotoxic T cell
Humans
Photosensitizer
Physical and Theoretical Chemistry
Carcinoma, Small Cell
Cytotoxicity
Cellular Debris
Subjects
Details
- ISSN :
- 00318655
- Volume :
- 63
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Photochemistry and photobiology
- Accession number :
- edsair.doi.dedup.....3547b6bb3170f0623a3f1906dcfea66b