Back to Search
Start Over
Deep tissue scattering compensation with three-photon F-SHARP
- Source :
- Optica
- Publication Year :
- 2021
-
Abstract
- Optical imaging techniques are widely used in biological research, but their penetration depth is limited by tissue scattering. Wavefront shaping techniques are able to overcome this problem in principle, but are often slow, and their performance depends on the sample. This greatly reduces their practicability for biological applications. Here we present a scattering compensation technique based on three-photon excitation, which converges faster than comparable two-photon (2P) techniques and works reliably even on densely labeled samples, where 2P approaches fail. To demonstrate its usability and advantages for biomedical imaging, we apply it to the imaging of dendritic spines on layer 5 neurons labeled with green fluorescent protein in an anesthetized mouse.
- Subjects :
- Wavefront
Physics
0303 health sciences
Photon
Scattering
business.industry
01 natural sciences
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Compensation (engineering)
010309 optics
03 medical and health sciences
Optics
Deep tissue
0103 physical sciences
Medical imaging
business
Penetration depth
Excitation
030304 developmental biology
Subjects
Details
- ISSN :
- 23342536
- Database :
- OpenAIRE
- Journal :
- Optica
- Accession number :
- edsair.doi.dedup.....35599df40c6e8a6706e1f01a693693e4
- Full Text :
- https://doi.org/10.1364/optica.440279