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Equine allogeneic chondrogenic induced mesenchymal stem cells: A GCP target animal safety and biodistribution study
- Source :
- Research in veterinary science. 117
- Publication Year :
- 2017
-
Abstract
- The safety of the intra-articular use of mesenchymal stem cells (MSCs) is scarcely reported. Therefore, the goal of this study was to investigate the safety of a single intra-articular injection with allogeneic chondrogenic induced MSCs combined with equine plasma (=the investigational product: IVP) compared to a saline (0.9% NaCl) placebo control (=control product: CP). Sixteen healthy experimental horses were randomly assigned to receive a single intra-articular injection with either the IVP (n=8) or the CP (n=8) in the left metacarpophalangeal joint. All horses underwent a daily clinical assessment throughout the entire study period of 42days to assess adverse events. Additionally, a local joint assessment and a lameness examination were performed daily during the first two weeks, and weekly the following 4weeks. Blood samples were taken weekly for hematological and biochemical analysis. At the end of the study period, horses of the IVP group were euthanized for a thorough necropsy and to check for biodistribution. Tissue samples of the injected joint were collected for histological examination. In both CP and IVP treated horses a mild transient subjective increase in periarticular temperature and lameness was noted after the intra-articular injection with no significant differences between the treatment groups. No distribution of the cells was found using immunohistochemistry and no ectopic tissue formation or signs of inflammation were found on histology. A single intra-articular injection of allogeneic chondrogenic induced MSCs combined with allogeneic plasma in horses had the same clinical side effects as an intra-articular injection with saline solution.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Pathology
medicine.medical_specialty
medicine.medical_treatment
Osteoarthritis
Placebo
Injections, Intra-Articular
03 medical and health sciences
Blood plasma
Medicine
Animals
Tissue Distribution
Horses
Saline
General Veterinary
business.industry
Mesenchymal stem cell
Histology
Mesenchymal Stem Cells
medicine.disease
030104 developmental biology
Lameness
Stem cell
Joint Diseases
business
Chondrogenesis
Subjects
Details
- ISSN :
- 15322661
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Research in veterinary science
- Accession number :
- edsair.doi.dedup.....35635c775f9f87e33f64d78fff78e165