Supplementary Figure 1 from Tumor Suppressive Effects of Bromodomain-Containing Protein 7 (BRD7) in Epithelial Ovarian Carcinoma

PDF - 1513K, BRD7 mediated tumor suppressive effects are not correlated with cell cycle. A, Cell cycle distributions of A2780 (marked as A) and SKOV3 (marked as S) cells measured by flow cytometry. Cell number histograms plot (upper panel) show the distribution of cells on the basis of DNA contents as determined by PI fluorescence intensity. Left peak represents cells in the G1/G0 phases, hatched area represents cells in S phase, and peak on right represents cells in the G2 and M phases. The expression levels of BRD7 mRNA among phases of cell cycle show no significant differences in both A2780 and SKOV3 cell line (.lower panel). Values are expressed as means of three independent experiments plus or minus SD. B, Western blot analysis was used to determine expression levels of Cyclin B1, D1 or E2.