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Combined treatment of melatonin and sodium tanshinone IIA sulfonate reduced the neurological and cardiovascular toxicity induced by deltamethrin in zebrafish
- Source :
- Chemosphere. 243
- Publication Year :
- 2019
-
Abstract
- The pyrethroid insecticide deltamethrin has been reported to have an effect on vertebrate development and cardiovascular disease. Sodium tanshinone IIA sulfonate (STS) is considered to have cardioprotective effects and melatonin is known to regulate sleep-waking cycles. In this experiment, we used transgenic zebrafish Tg (kdrl:mCherry) and Tg (myl7:GFP) to investigate whether STS and melatonin could reverse the cardiovascular toxicity and neurotoxicity induced by deltamethrin. Zebrafish embryos were exposed to 25 μg/L deltamethrin at 10 hpf and treated with 100 mmol/L STS and 1 μmol/L melatonin showed that deltamethrin treatment affected normal cardiovascular development. In situ hybridization and qRT-PCR results showed that deltamethrin could interfere with the normal expression of cardiovascular development-related genes vegfr2, shh, gata4, nkx2.5, causing functional defects in the cardiovascular system. In addition, deltamethrin could affect the sleep-waking behavior of larvae, increasing the activity of larvae, decreasing the rest behavior and the expression of hcrt, hcrtr, aanat2 were down-regulated. The addition of melatonin and STS can significantly alleviate cardiovascular toxicity and sleep-waking induced by deltamethrin, while restoring the expression of related genes to normal levels. Our study demonstrates the role of STS and melatonin in protecting cardiovascular and sleep-waking behavior caused by deltamethrin.
- Subjects :
- Environmental Engineering
Health, Toxicology and Mutagenesis
0208 environmental biotechnology
Down-Regulation
02 engineering and technology
In situ hybridization
010501 environmental sciences
Pharmacology
01 natural sciences
Cardiovascular System
Nervous System
Melatonin
Animals, Genetically Modified
chemistry.chemical_compound
parasitic diseases
Nitriles
Pyrethrins
medicine
Environmental Chemistry
Animals
Pesticides
Zebrafish
0105 earth and related environmental sciences
Cardiotoxicity
Orexins
biology
Chemistry
GATA4
Public Health, Environmental and Occupational Health
Neurotoxicity
General Medicine
General Chemistry
Phenanthrenes
biology.organism_classification
medicine.disease
Pollution
020801 environmental engineering
Deltamethrin
Larva
MYL7
medicine.drug
Subjects
Details
- ISSN :
- 18791298
- Volume :
- 243
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....35b437ab2e59091113c1cac477605fb4