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Role of complement anaphylatoxin receptors (C3aR, C5aR) in the development of the rat cerebellum
- Source :
- Molecular Immunology, Molecular Immunology, Elsevier, 2008, 45 (14), pp.3767-3774. ⟨10.1016/j.molimm.2008.05.027⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- International audience; There is now strong evidence for non-immune or inflammatory functions of complement, notably in the central nervous system. In particular, it has been recently reported that the anaphylatoxin receptors C3aR and C5aR are transiently expressed in the cerebellar cortex of newborn rat, suggesting that anaphylatoxins are involved in the histogenesis of the cerebellum. In the present study, we have investigated the effects of C3aR and C5aR agonists and antagonists on the development of the cerebellum of 11-12-day-old rats in vivo and in vitro. Sub-dural injection of C3aR and C5aR agonists at the surface of the cerebellum transiently modified the thickness of the cortical layers. The C5aR agonist provoked an enlargement of the external granule cell layer (EGL) that was due to increased proliferation of immature granule neurons. Conversely, the C3aR agonist decreased the thickness of the EGL and increased the thickness of the internal granule cell layer (IGL), suggesting that C3a accelerates the migration process of granule cells from the EGL to the IGL. Video-microscopy examination of cultured granule neurons confirmed the role of C3aR in cell motility. These results provide clear evidence for the involvement of anaphylatoxin receptors in the histogenesis of the cerebellar cortex.
- Subjects :
- Cerebellum
Anaphylatoxins
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Neurons
Apoptosis
MESH: Receptors, G-Protein-Coupled
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
Receptors, G-Protein-Coupled
0302 clinical medicine
Cell Movement
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Animals
MESH: Receptor, Anaphylatoxin C5a
MESH: Cell Movement
Cells, Cultured
Neurons
0303 health sciences
MESH: Anaphylatoxins
Immunohistochemistry
Cell biology
Receptors, Complement
medicine.anatomical_structure
CXCL3
Cerebellar cortex
MESH: Cells, Cultured
Agonist
MESH: Rats
medicine.drug_class
Immunology
Central nervous system
Biology
03 medical and health sciences
MESH: Cell Proliferation
medicine
Animals
Anaphylatoxin
Rats, Wistar
Molecular Biology
Receptor, Anaphylatoxin C5a
030304 developmental biology
Cell Proliferation
Anaphylatoxin receptors
MESH: Apoptosis
MESH: Immunohistochemistry
MESH: Rats, Wistar
Granule cell
MESH: Cerebellum
Rats
MESH: Receptors, Complement
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 01615890
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology, Molecular Immunology, Elsevier, 2008, 45 (14), pp.3767-3774. ⟨10.1016/j.molimm.2008.05.027⟩
- Accession number :
- edsair.doi.dedup.....35b9816fc1f8d609cc9c8d287c597b52