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CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in ‘Newhall’ orange
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2016
- Publisher :
- Oxford University Press, 2016.
-
Abstract
- Highlight CitAP2.10 is a novel AP2/ERF transcription factor associated with (+)-valencene content in sweet orange fruit and operates by modulating CsTPS1 transcript abundance.<br />Aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, and characteristic volatiles have been analyzed in different citrus species. In sweet orange, Citrus sinensis, the sesquiterpene (+)-valencene is a key volatile compound in the fruit peel. Valencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, is characterized as a regulator of (+)-valencene synthesis. The expression pattern of CitAP2.10 was positively correlated with (+)-valencene content and CsTPS1 expression. Dual-luciferase assays indicated that CitAP2.10 could trans-activate the CsTPS1 promoter. Ethylene enhanced expression of CitAP2.10 and this effect was abolished by the ethylene antagonist 1-methylcyclopropene. The role and function of CitAP2.10 in (+)-valencene biosynthesis were confirmed using the Arabidopsis homolog (AtWRI1), which also transiently activated the CsTPS1 promoter. Furthermore, transient over-expression of CitAP2.10 triggered (+)-valencene biosynthesis in sweet orange fruit. These results indicate that CitAP2.10 regulates (+)-valencene synthesis via induction of CsTPS1 mRNA accumulation.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Ethylene
Physiology
education
terpene synthase
Plant Science
Orange (colour)
TPS
Real-Time Polymerase Chain Reaction
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Valencene
Gene Expression Regulation, Plant
Arabidopsis
citrus volatile
Gene expression
ethylene
transcriptional regulation
valencene
Promoter Regions, Genetic
Transcription factor
Alkyl and Aryl Transferases
biology
Arabidopsis Proteins
food and beverages
biology.organism_classification
Enzyme Activation
sweet orange
030104 developmental biology
chemistry
Biochemistry
CitAP2/ERF
Fruit
Sesquiterpenes
Citrus × sinensis
010606 plant biology & botany
Research Paper
Citrus sinensis
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 14602431 and 00220957
- Volume :
- 67
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....35c142f983fd1d7b90c7a9806092df70