Additional file 1 of Angiogenesis in mammary Paget disease: histopathological analyses of blood vessel density and angiogenic factors

Additional file 1: Supplemental Figure 1. Immunohistochemical double staining of CD34 (red) and Ki-67 (brown). Red cells are CD34-positive endothelial cells. The endothelial cells with brown nuclei are considered to be proliferating (arrow). (A) In a control case, only one brown nucleus in an endothelial cell is observed. (B) In a case of mammary Paget disease, many proliferating endothelial cells are observed. Epidermal basal layer cells are used as a positive internal control. All magnifications: × 400.