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Bone Cell Autophagy Is Regulated by Environmental Factors
- Source :
- Cells Tissues Organs. 194:274-278
- Publication Year :
- 2011
- Publisher :
- S. Karger AG, 2011.
-
Abstract
- The goal of this investigation was to ascertain whether bone cells undergo autophagy and to determine if this process is regulated by environmental factors. We showed that osteocytes in both murine and human cortical bone display a punctuate distribution of microtubule-associated protein light chain 3, indicative of autophagy. In addition, we noted a basal level of autophagy in preosteocyte-like murine long bone-derived osteocytic (MLO)-A5 cells. Autophagy was upregulated following nutrient deprivation and hypoxic culture, stress conditions that osteocytes encounter in vivo. Furthermore, in response to calcium stress, the transcription factor hypoxia inducible factor 1 regulated MLO-A5 autophagy. Finally, we showed that the more differentiated MLO-Y4 osteocyte-like cells exhibited a significant basal autophagic flux. Based on these findings, we suggest that raising the level of autophagic flux is a mechanism by which differentiated bone cells survive in a stressful environment.
- Subjects :
- Paper
Histology
Thapsigargin
Environment
Biology
Osteocytes
Bone and Bones
Cell Line
Mice
chemistry.chemical_compound
Downregulation and upregulation
Bone cell
Autophagy
medicine
Animals
Humans
Rats, Wistar
Transcription factor
G alpha subunit
Hypoxia-Inducible Factor 1, alpha Subunit
Cell Hypoxia
Rats
Cell biology
Phenotype
medicine.anatomical_structure
chemistry
Cell culture
Cortical bone
Anatomy
Microtubule-Associated Proteins
Subjects
Details
- ISSN :
- 14226421 and 14226405
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Cells Tissues Organs
- Accession number :
- edsair.doi.dedup.....361b92510c43e5c464b5216cb05a3995