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Fast and highly selective determination of hemagglutinin content in quadrivalent influenza vaccine by reversed-phase high-performance liquid chromatography method
- Source :
- Journal of Chromatography A. 1528:18-24
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Seasonal inactivated quadrivalent influenza vaccines are currently formulated to include antigens from two strains of influenza A and a strain from each of the two circulating influenza B virus lineages. However, the applicability of the potency assay currently required for the release of vaccines has been hindered due to cross-reactivity between the two B strains. In this study, a reversed-phase high-performance liquid chromatography method previously developed for the separation and quantitative determination of the hemagglutinin content in trivalent influenza vaccine preparations was further extended and found to be adaptable for the assessment of all four hemagglutinin antigens present in quadrivalent influenza vaccines. Vaccines prepared from monovalent bulks and commercial quadrivalent products from the past three vaccination seasons in the Northern Hemisphere were tested with the new method. The results showed excellent resolution of all four hemagglutinins from frequently interfering formulation agents such as surfactants. This method provides a simple approach for fast evaluation of quality and hemagglutinin strain identification in influenza vaccines. It is also the only physicochemical method capable of distinguishing the B strains in quadrivalent influenza vaccines.
- Subjects :
- 0301 basic medicine
Trivalent influenza vaccine
Chemistry, Pharmaceutical
Hemagglutinin (influenza)
medicine.disease_cause
Biochemistry
High-performance liquid chromatography
Virus
Analytical Chemistry
03 medical and health sciences
0302 clinical medicine
Antigen
Influenza A virus
medicine
030212 general & internal medicine
Chromatography, Reverse-Phase
Chromatography
biology
Chemistry
Strain (biology)
Organic Chemistry
General Medicine
Vaccination
Influenza B virus
Hemagglutinins
030104 developmental biology
Influenza Vaccines
biology.protein
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 1528
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....36283f3dc76b197f03cdaf067671da93