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Bufalin Induces Apoptosis and Improves the Sensitivity of Human Glioma Stem-Like Cells to Temozolamide
- Source :
- Oncology Research
- Publication Year :
- 2019
- Publisher :
- Computers, Materials and Continua (Tech Science Press), 2019.
-
Abstract
- Glioma is the most common malignant tumor of the central nervous system, and it is characterized by high relapse and fatality rates and poor prognosis. Bufalin is one of the main ingredients of Chan-su, a traditional Chinese medicine (TCM) extracted from toad venom. Previous studies revealed that bufalin exerted inhibitory effects on a variety of tumor cells. To demonstrate the inhibitory effect of bufalin on glioma cells and glioma stem-like cells (GSCs) and discuss the underlying mechanism, the proliferation of glioma cells was detected by MTT and colony formation assays following treatment with bufalin. In addition, we investigated whether bufalin inhibits or kills GSCs using flow cytometry, Western blotting, and reverse transcription polymerase chain reaction analysis (RT-PCR). Finally, we investigated whether bufalin could improve the therapeutic effect of temozolomide (TMZ) and discussed the underlying mechanism. Taken together, our data demonstrated that bufalin inhibits glioma cell growth and proliferation, inhibits GSC proliferation, and kills GSCs. Bufalin was found to induce the apoptosis of GSCs by upregulating the expression of the apoptotic proteins cleaved caspase 3 and poly(ADP-ribose) polymerase (PARP) and by downregulating the expression of human telomerase reverse transcriptase, which is a marker of telomerase activity. Bufalin also improved the inhibitory effect of TMZ on GSCs by activating the mitochondrial apoptotic pathway. These results suggest that bufalin damages GSCs, induces apoptosis, and enhances the sensitivity of GSCs to TMZ.
- Subjects :
- 0301 basic medicine
endocrine system
Cancer Research
Telomerase
Poly ADP ribose polymerase
Apoptosis
Antineoplastic Agents
Caspase 3
Article
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Glioma
Temozolomide
medicine
Humans
Telomerase reverse transcriptase
Cell Proliferation
Chemistry
Bufalin
General Medicine
Cancer stem-like cells
medicine.disease
Temozolomide (TMZ)
Bufanolides
030104 developmental biology
Oncology
Drug Resistance, Neoplasm
Cell culture
030220 oncology & carcinogenesis
Neoplastic Stem Cells
Cancer research
Subjects
Details
- ISSN :
- 09650407
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics
- Accession number :
- edsair.doi.dedup.....3648e2d224a3b941666841ef18504c94