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Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection.
- Subjects :
- 0301 basic medicine
medicine.drug_class
media_common.quotation_subject
030106 microbiology
Antibiotics
Virus Replication
Article
Microbiology
03 medical and health sciences
Viral entry
Amphotericin B
Cell Line, Tumor
Enterovirus Infections
medicine
Enterovirus 71
Humans
Internalization
media_common
Multidisciplinary
biology
HEK 293 cells
Virus Internalization
biology.organism_classification
Virology
Enterovirus A, Human
030104 developmental biology
Viral replication
Cell culture
medicine.drug
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3652dec9e8115322e8104d4d86123f87