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Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells’ Proliferation through Regulating the Cell Cycle
- Source :
- International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 271, International Journal of Molecular Sciences, Vol 19, Iss 1, p 271 (2018), International Journal of Molecular Sciences
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Notch signaling as a conserved cell fate regulator is involved in the regulation of cell quiescence, proliferation, differentiation and postnatal tissue regeneration. However, how Notch signaling regulates porcine satellite cells (PSCs) has not been elucidated. We stably transfected Notch1 intracellular domain (N1ICD) into PSCs to analyze the gene expression profile and miRNA-seq. The analysis of the gene expression profile identified 295 differentially-expressed genes (DEGs) in proliferating-N1ICD PSCs (P-N1ICD) and nine DEGs on differentiating-N1ICD PSCs (D-N1ICD), compared with that in control groups (P-Control and D-Control, respectively). Analyzing the underlying function of DEGs showed that most of the upregulated DEGs enriched in P-N1ICD PSCs are related to the cell cycle. Forty-four and 12 known differentially-expressed miRNAs (DEMs) were identified in the P-N1ICD PSCs and D-N1ICD PSCs group, respectively. Furthermore, we constructed the gene-miRNA network of the DEGs and DEMs. In P-N1ICD PSCs, miR-125a, miR-125b, miR-10a-5p, ssc-miR-214, miR-423 and miR-149 are downregulated hub miRNAs, whose corresponding hub genes are marker of proliferation Ki-67 (MKI67) and nuclear receptor binding SET domain protein 2 (WHSC1). By contrast, miR-27a, miR-146a-5p and miR-221-3p are upregulated hub miRNAs, whose hub genes are RUNX1 translocation partner 1 (RUNX1T1) and fibroblast growth factor 2 (FGF2). All the hub miRNAs and genes are associated with cell proliferation. Quantitative RT-PCR results are consistent with the gene expression profile and miRNA-seq results. The results of our study provide valuable information for understanding the molecular mechanisms underlying Notch signaling in PSCs and skeletal muscle development.
- Subjects :
- pig
0301 basic medicine
muscle satellite cells
Satellite Cells, Skeletal Muscle
proliferation
Sus scrofa
mRNA-seq
Notch signaling pathway
N1ICD
miRNA-seq
Cell fate determination
Biology
Article
Catalysis
Cell Line
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
Cell quiescence
microRNA
Gene expression
Animals
Gene Regulatory Networks
RNA, Messenger
Receptor, Notch1
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Cell Proliferation
Base Sequence
Sequence Analysis, RNA
Cell growth
Cell Cycle
Organic Chemistry
RUNX1T1
Reproducibility of Results
General Medicine
Cell cycle
Computer Science Applications
Cell biology
MicroRNAs
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Transcriptome
Transcription Factors
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....365cc13262f53970f08d30360a3bbb45