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Effect of Bisphenol A on Rat Metabolic Profiling Studied by Using Capillary Electrophoresis Time-of-Flight Mass Spectrometry
- Source :
- Environmental Science & Technology. 47:7457-7465
- Publication Year :
- 2013
- Publisher :
- American Chemical Society (ACS), 2013.
-
Abstract
- Bisphenol A (BPA), a chemical widely used in the manufacture of polycarbonate plastics, has raised considerable concern in recent decades because of its hormone-like properties. Whether BPA exposure is a health risk remains controversial in many countries. A metabolomics study based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOF/MS) was performed to study the urine metabolic profiles of Sprague-Dawley rats fed with four dose levels of BPA (0, 1, 10, and 100 μg/kg body weight) for 45 days. Multivariate pattern recognition directly reflected the metabolic perturbations caused by BPA. On the basis of univariate analysis, 42 metabolites including amino acids, polyamines, nucleosides, organic acids, carbohydrates, pterins, polyphenols, and sugar phosphates were found as the most significantly differential metabolites. The marked perturbations were related with valine, leucine and isoleucine biosynthesis, D-glutamine and D-glutamate metabolism, etc. Significant alterations of neurotransmitters (glutamate, gamma-aminobutyric acid, and noradrenaline) and neurotransmitter-related metabolites (tyrosine, histamine, valine, and taurine) suggested that the toxic effects of small-dose BPA (below 50 mg/kg/day) may contribute to its interactions with the neuromediating system. Our study demonstrated that metabolomics may offer more specific insights into the molecular changes underlying the physiological effects of BPA.
- Subjects :
- Male
endocrine system
Bisphenol A
Glutamic Acid
Endocrine Disruptors
Mass Spectrometry
Rats, Sprague-Dawley
Norepinephrine
chemistry.chemical_compound
Metabolomics
Capillary electrophoresis
Phenols
Valine
Animals
Environmental Chemistry
Estrogens, Non-Steroidal
Benzhydryl Compounds
chemistry.chemical_classification
Neurotransmitter Agents
Sugar phosphates
Electrophoresis, Capillary
General Chemistry
Metabolism
Rats
Amino acid
chemistry
Biochemistry
Metabolome
Leucine
Subjects
Details
- ISSN :
- 15205851 and 0013936X
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Environmental Science & Technology
- Accession number :
- edsair.doi.dedup.....36701d309defae914ddf86b65b713200