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Non-specific oligodendrocyte cytotoxicity mediated by soluble products of activated T cell lines
- Source :
- Journal of Neuroimmunology. 35:261-271
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- Supernates from myelin basic protein (MBP)-reactive T cell lines have been tested by a battery of assays for cytotoxicity against oligodendrocytes in vitro. All supernates tested demonstrated cytotoxic activity in both a dose- and time-dependent manner that ranged from 29.1% to 55.8% after 48 h incubation. Oligodendrocyte cytotoxicity mediated by MBP-reactive T cell lines was not antigen specific since lines reactive with purified protein derivative (PPD) of tuberculin showed similar cytotoxicity. Supernates cytotoxic to oligodendrocytes were equally effective against syngeneic and non-syngeneic target cells, but had no effect upon astrocytes. Neutralization studies revealed that oligodendrocyte cytotoxicity mediated by MBP-reactive T cell supernates could only be partially attributed to lymphotoxin/tumor necrosis factor activity, and was not associated with perforin, serine proteinase or N-methyl-D-aspartate (NMDA) receptor agonists.
- Subjects :
- Cytotoxicity, Immunologic
T-Lymphocytes
T cell
Immunology
Lymphocyte Activation
Cell Line
Mice
Neutralization Tests
medicine
Animals
Immunology and Allergy
Cytotoxic T cell
Cytotoxicity
biology
Myelin Basic Protein
T lymphocyte
Molecular biology
Oligodendrocyte
Myelin basic protein
Oligodendroglia
medicine.anatomical_structure
Lymphotoxin
Solubility
Neurology
Perforin
Astrocytes
biology.protein
Female
Neurology (clinical)
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....367191da8b1b36e38cea471e2a4344e9