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Influence of changes in the intestinal microflora on the immune function in mice
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2018
- Publisher :
- 日本獣医学会, 2018.
-
Abstract
- The composition of the intestinal microbiota is related to the health and immune function of the host. Administration of antibiotics affects the composition of the intestinal microbiota. However, the effects of immune function on the composition of the intestinal microbiota are still unclear. In this study, we investigated the lymphocyte composition and determined the relationships between lymphocyte function and the intestinal microbiota following antibiotic treatment in mice. To change the composition of the intestinal microbiota, mice were treated with or without antibiotics. Analysis of intestinal microbiota was performed by metagenomic analysis targeting 16S rRNA. Lymphocyte subsets of splenocytes were measured by flow cytometry. For functional analysis of T cells, splenocytes were stimulated with concanavalin (Con A), and cytokine gene expression was measured by real-time polymerase chain reaction. Firmicutes were predominant in the control group, whereas Bacteroidetes predominated in the antibiotic-treated group, as determined by metagenomic analysis. The diversity of the microbiota decreased in the antibiotic-treated group. Analysis of lymphocyte subsets showed that CD3(+) cells decreased, whereas CD19(+) cells increased in the antibiotic-treated group. All cytokine genes in splenocytes treated with Con A were downregulated in the antibiotic-treated group; in particular, genes encoding interferon-gamma, interleukin (IL)-6, and IL-13 significantly decreased. Taken together, these results revealed that changes in the composition of the intestinal microbiota by antibiotic treatment influenced the population of lymphocytes in splenocytes and affected the immune response.
- Subjects :
- 0301 basic medicine
intestinal microbiota
medicine.drug_class
medicine.medical_treatment
T cell
Lymphocyte
Immunology
Antibiotics
Population
Real-Time Polymerase Chain Reaction
immune response
Microbiology
Mice
03 medical and health sciences
Immune system
T-Lymphocyte Subsets
RNA, Ribosomal, 16S
Concanavalin A
medicine
cytokine
Animals
education
Mice, Inbred BALB C
education.field_of_study
Full Paper
General Veterinary
biology
metagenomics analysis
Immunity
Interleukin
Flow Cytometry
Anti-Bacterial Agents
Gastrointestinal Microbiome
030104 developmental biology
Cytokine
medicine.anatomical_structure
biology.protein
Cytokines
Spleen
Subjects
Details
- Language :
- English
- Volume :
- 80
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Medical Science
- Accession number :
- edsair.doi.dedup.....368457cb00018b97049c00cba2cbdb5a