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Metabolite profiling of polyphenols in peels of Citrus limetta Risso by combination of preparative high-speed countercurrent chromatography and LC–ESI–MS/MS
- Source :
- Food Chemistry. 158:139-152
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- The polar constituents of peels from Citrus limetta variety Risso (Rutaceae) were investigated by a combination of two complementary chromatographic techniques consisting of preparative high-speed countercurrent chromatography (HSCCC), and off-line LC-ESI-MS/MS analysis to design a two-dimensional metabolite profile. Countercurrent chromatography (CCC) using solely immiscible solvent systems allowed the fractionation of principal components and an enrichment of minor concentrated metabolites from a crude polar solvent partition of C. limetta peels for subsequent structural identification by LC-ESI-MS/MS analysis. The combination of two very different chromatographic techniques resulted in lower detection limits for electrospray mass-spectrometry and revealed eighty-five compounds, including three abscisic acid derivatives, five limonoid glycosides, twenty-six dihydro-cinnamic and cinnamic acid glycosides, eleven flavanone glycosides, seven flavone glycosides, seventeen flavonol glycosides, including limocitrol and limocitrin derivatives. As a chemocharacteristic for C. limetta metabolites, many of the detected structures were linked to single and multiple 3-hydroxy-3-methyl-glutaryl (HMG) substitutions. C. limetta peels are a by-product of juice production, and not only the antioxidant fractions but also some of the fortified compounds could be used for food and pharmaceutical purposes.
- Subjects :
- chemistry.chemical_classification
Citrus
Chromatography
Flavonols
Metabolite
Polyphenols
Glycoside
General Medicine
Limonoid
Citrus limetta
Mass Spectrometry
Cinnamic acid
Analytical Chemistry
chemistry.chemical_compound
Countercurrent chromatography
chemistry
Polyphenol
medicine
Organic chemistry
Glycosides
Countercurrent Distribution
Flavanone
Chromatography, Liquid
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 03088146
- Volume :
- 158
- Database :
- OpenAIRE
- Journal :
- Food Chemistry
- Accession number :
- edsair.doi.dedup.....36a96e40aa8aedb5c24f640600aa9d4d