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Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer
- Source :
- Oncotarget
- Publication Year :
- 2017
- Publisher :
- Impact Journals LLC, 2017.
-
Abstract
- Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa.
- Subjects :
- 0301 basic medicine
Gerontology
Adult
Male
proliferation
Gene Expression
Kinesins
Apoptosis
medicine.disease_cause
urologic and male genital diseases
Transcriptome
03 medical and health sciences
Prostate cancer
0302 clinical medicine
DU145
KIF14
Cell Line, Tumor
medicine
Biomarkers, Tumor
Humans
Aged
Cell Proliferation
Neoplasm Staging
Oncogene Proteins
Oncogene
G2 arrest
business.industry
Gene Expression Profiling
Cancer
Computational Biology
Prostatic Neoplasms
Oncogenes
Middle Aged
medicine.disease
prostate cancer
Prognosis
G2 Phase Cell Cycle Checkpoints
030104 developmental biology
Oncology
Tumor progression
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Cancer research
Disease Progression
Neoplasm Grading
Carcinogenesis
business
GADD45A
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19492553
- Volume :
- 8
- Issue :
- 28
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....36d1e9b183c28ca9ebb78a4ec7f27ba8