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Safety evaluation of the food enzyme triacylglycerol lipase from the genetically modified Aspergillus niger strain NZYM‐DB
- Source :
- EFSA Journal, Vol 19, Iss 1, Pp n/a-n/a (2021), RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname, EFSA Journal
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase EC 3.1.1.3) is produced with a genetically modified Aspergillus niger strain NZYM‐DB by Novozymes A/S. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and recombinant DNA. The food enzyme is intended to be used in an immobilised form in the production of modified fats and oils by interesterification. Based on the estimated use levels recommended for interesterification of fats and oils and individual data from the EFSA Comprehensive European Food Database, dietary exposure to the food enzyme–total organic solids (TOS) was estimated to be up to 0.75 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not raise a safety concern. The systemic toxicity was assessed by means of a repeated dose 90‐day oral toxicity study in rats. The Panel identified a no observed adverse effect level at the highest dose of 1,132 mg TOS/kg bw per day, which when compared with the estimated dietary exposure, results in a margin of exposure of at least 1,500. Similarity of the amino acid sequence to those of known allergens was searched and no match was found. The Panel considered that, under the intended conditions of use, the risk of allergic sensitisation and elicitation reactions by dietary exposure cannot be excluded, but the likelihood of such reactions to occur is likely to be low. Based on the data provided, the immobilisation of the food enzyme and the removal of total organic solids during fats and oils processing, the Panel concluded that the food enzyme does not give rise to safety concerns under the intended conditions of use.
- Subjects :
- No-observed-adverse-effect level
TECNOLOGIA DE ALIMENTOS
040301 veterinary sciences
Interesterified fat
Veterinary (miscellaneous)
Triacylglycerol lipase
TP1-1185
Plant Science
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Microbiology
Triacylglycerol acylhydrolase
0403 veterinary science
medicine
Food enzyme
TX341-641
Food science
Lipase
0105 earth and related environmental sciences
chemistry.chemical_classification
Genetically modified microorganism
biology
Nutrition. Foods and food supply
Chemical technology
Aspergillus niger
04 agricultural and veterinary sciences
biology.organism_classification
Genetically modified organism
Scientific Opinion
Enzyme
chemistry
biology.protein
Animal Science and Zoology
Parasitology
Genotoxicity
EC 3.1.1.3
Food Science
Subjects
Details
- ISSN :
- 18314732
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- EFSA Journal
- Accession number :
- edsair.doi.dedup.....36d5399aeb046600d3d678bee9a55216