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KRAS mutations testing in non-small cell lung cancer: the role of Liquid biopsy in the basal setting
- Source :
- J Thorac Dis
- Publication Year :
- 2020
- Publisher :
- AME Publishing Company, 2020.
-
Abstract
- In advanced stage non-small cell lung cancer (NSCLC) patients, Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) testing may soon acquire a predictive significance to select patients for AMG510 treatment. Since tissue samples are not always available, liquid biopsy may represent a viable option for KRAS testing. Here, we review the last three years clinical practice performed on 194 plasma based liquid biopsies by next generation sequencing (NGS) SiRe(®) panel. In particular, 36 (18.6%) KRAS mutated cases were identified, with an overall median allelic frequency of 5.0% (ranging between 0.2% and 46.8%). No concomitant mutations were observed in the other NSCLC clinical relevant genes included in the SiRe(®) panel, such as epidermal growth factor receptor (EGFR) and v-Raf murine sarcoma viral oncogene homolog B (BRAF). Exon 2 p.G12C was the most common detected mutation (13/36, 36.1%). In conclusion, our data update and confirm that SiRe(®) NGS panel represents a robust analytical tool to assess KRAS mutational status on circulating tumor DNA. Further investigation is required to design more cost-effective diagnostic algorithms to harmonize clinical relevant biomarker testing on tissue and blood in advanced stage NSCLC clinical practice.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
AMG510
Settore MED/06 - Oncologia Medica
Viral Oncogene
medicine.disease_cause
03 medical and health sciences
Basal (phylogenetics)
0302 clinical medicine
G12C
Medicine
Epidermal growth factor receptor
Liquid biopsy
Lung cancer
neoplasms
Mutation
biology
business.industry
Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS)
Review Article on Improving Outcomes in Lung Cancer Through Early Diagnosis and Smoking Cessation
medicine.disease
Basal setting
030104 developmental biology
Next generation sequencing (NGS)
030220 oncology & carcinogenesis
Cancer research
biology.protein
Biomarker (medicine)
KRAS
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- J Thorac Dis
- Accession number :
- edsair.doi.dedup.....36fea6f163ebed72a0d40adaae3229c4