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TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp
- Source :
- Developmental and comparative immunology. 121
- Publication Year :
- 2021
-
Abstract
- TRIF is an antiviral adaptor downstream of Toll-like receptors, the roles of teleost TRIF and their regulation remain largely unknown. In this study, a TRIF homologue (bcTRIF) of black carp (Mylopharyngodon piceus) has been cloned, and the transcription of bcTRIF in vivo and ex vivo increased in response to different stimuli. Overexpressed bcTRIF induced the transcription of interferon promoter in the EPC cells and enhanced protection of cells against infection of spring viremia of carp virus (SVCV). The previous study has identified that black carp TRAF2 (bcTRAF2) and TRAF6 (bcTRAF6) functioned positively in RIG-I/MAVS signaling. When co-expressed with bcTRAF2, bcTRIF-induced the transcription of interferon promoter in EPC cells was decreased, and the antiviral activity of bcTRIF was dampened accordingly. On the contrary, co-expressed bcTRAF6 enhanced both bcTRIF-mediated interferon promoter transcription and antiviral activity. The subsequent co-immunoprecipitation identified the interaction between bcTRAF2/6 and bcTRIF. Thus, bcTRIF-mediated antiviral signaling is up-regulated by bcTRAF6 and down-regulated by bcTRAF2.
- Subjects :
- 0301 basic medicine
Fish Proteins
TRAF2
Carps
Immunology
Down-Regulation
Virus
03 medical and health sciences
Fish Diseases
0302 clinical medicine
Black carp
Transcription (biology)
Interferon
medicine
Animals
Cloning, Molecular
Receptors, Immunologic
Carp
TNF Receptor-Associated Factor 6
biology
biology.organism_classification
TNF Receptor-Associated Factor 2
Cell biology
Up-Regulation
Adaptor Proteins, Vesicular Transport
030104 developmental biology
TRIF
Ex vivo
030215 immunology
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 18790089
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....3704d516975953b624f7fc1f08aea513