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Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell
- Source :
- Carbohydrate Polymers. 96:516-521
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48 h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MTT method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and IFN-γ production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment.
- Subjects :
- Polymers and Plastics
T-Lymphocytes
medicine.medical_treatment
T cell
Lymphocyte proliferation
Biology
Interferon-gamma
Mice
Antigen
Polysaccharides
Materials Chemistry
medicine
Animals
Immunologic Factors
Cell Proliferation
Antigen Presentation
Organic Chemistry
Dendritic Cells
Dendritic cell
T lymphocyte
Rehmannia glutinosa
biology.organism_classification
Rehmannia
medicine.anatomical_structure
Cytokine
Immunology
Interleukin-2
Bone marrow
Subjects
Details
- ISSN :
- 01448617
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Polymers
- Accession number :
- edsair.doi.dedup.....370fb913a31de926a0afac5ffc4cbdec