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Plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in Morocco
- Source :
- Journal of Infection in Developing Countries, Journal of Infection in Developing Countries, Independent, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩, Journal of Infection in Developing Countries, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- Introduction : Although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or AmpC-type beta-lactamases. Since no data are available in African countries, the prevalence of qnr genes at the University Hospital Ibn Rochd, Casablanca, Morocco, was investigated. Methodology: Between October 2006 and March 2007, the following 39 randomly selected non-duplicate Enterobacteriaceae producing an extended-spectrum beta-lactamase (ESBL), representing 20% of ESBL strains with respect to species and ward origin, were collected: Escherichia coli (n = 16); Klebsiella spp (n = 14); Enterobacter cloacae (n = 8); Proteus mirabilis (n = 1). Antibiotic susceptibility testing was performed according to CLSI guidelines. ESBL detection was performed by the double disc diffusion test. A multiplex PCR was conducted to detect qnrA , qnrB and qnrS genes that were confirmed by sequencing of the PCR product. Results: The estimated overall prevalence of qnr reached 36% (n = 14; qnrA , 10.25%; qnrB , 23.07%; qnrS , 2.56%). Genes were identified in E. coli , Klebsiella and Enterobacter with a respective prevalence of 18.7%, 50% and 62.5%. The qnr genes were detected in nine wards and qnrA1 , qnrB1-B2-B4 and qnrS1 variants were identified. Three genes were identified among nalidixic acid susceptible strains (n = 6); three of those were also susceptible to ciprofloxacin. Among nalidixic acid and ciprofloxacin resistant strains, all strains had qnrB . Conclusions: This study highlights the high prevalence of qnr genes among ESBL strains in the Ibn Rochd CHU, Casablanca. Moreover, qnr were present in quinolone-susceptible strains which could lead to in vivo selection of ciprofloxacin-resistant strains.
- Subjects :
- Klebsiella
Nalidixic acid
medicine.medical_treatment
MESH: beta-Lactamases
Quinolones
Polymerase Chain Reaction
Hospitals, University
0302 clinical medicine
Prevalence
030212 general & internal medicine
MESH: Enterobacteriaceae Infections
0303 health sciences
MESH: Microbial Sensitivity Tests
biology
Enterobacteriaceae Infections
General Medicine
Enterobacter
Enterobacteriaceae
3. Good health
Anti-Bacterial Agents
Ciprofloxacin
Morocco
Infectious Diseases
medicine.drug
Plasmids
Microbial Sensitivity Tests
Microbiology
beta-Lactamases
03 medical and health sciences
MESH: Enterobacteriaceae
MESH: Plasmids
Virology
MESH: Anti-Bacterial Agents
Drug Resistance, Bacterial
MESH: Drug Resistance, Bacterial
medicine
Humans
MESH: Prevalence
MESH: Hospitals, University
MESH: Humans
MESH: Quinolones
030306 microbiology
MESH: Polymerase Chain Reaction
biology.organism_classification
Proteus mirabilis
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Morocco
Beta-lactamase
Parasitology
Enterobacter cloacae
Subjects
Details
- Language :
- English
- ISSN :
- 20366590 and 19722680
- Database :
- OpenAIRE
- Journal :
- Journal of Infection in Developing Countries, Journal of Infection in Developing Countries, Independent, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩, Journal of Infection in Developing Countries, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩
- Accession number :
- edsair.doi.dedup.....371711d7d9af19136bd94516aa6657ec