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The erythropoietin receptor expressed in skeletal muscle is essential for mitochondrial biogenesis and physiological exercise
- Source :
- Pflugers Archiv, Pflügers Archiv : European Journal of Physiology. SPRINGER
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Erythropoietin (EPO) is a haematopoietic hormone that regulates erythropoiesis, but the EPO-receptor (EpoR) is also expressed in non-haematopoietic tissues. Stimulation of the EpoR in cardiac and skeletal muscle provides protection from various forms of pathological stress, but its relevance for normal muscle physiology remains unclear. We aimed to determine the contribution of the tissue-specific EpoR to exercise-induced remodelling of cardiac and skeletal muscle. Baseline phenotyping was performed on left ventricle and m. gastrocnemius of mice that only express the EpoR in haematopoietic tissues (EpoR-tKO). Subsequently, mice were caged in the presence or absence of a running wheel for 4 weeks and exercise performance, cardiac function and histological and molecular markers for physiological adaptation were assessed. While gross morphology of both muscles was normal in EpoR-tKO mice, mitochondrial content in skeletal muscle was decreased by 50%, associated with similar reductions in mitochondrial biogenesis, while mitophagy was unaltered. When subjected to exercise, EpoR-tKO mice ran slower and covered less distance than wild-type (WT) mice (5.5 ± 0.6 vs. 8.0 ± 0.4 km/day, p
- Subjects :
- Male
Cardiac function curve
medicine.medical_specialty
Muscle Physiology
Physiology
PGC-1-ALPHA
Clinical Biochemistry
PROTEIN
Exercise-induced physiological adaptation
Neovascularization, Physiologic
Erythropoietin receptor
CAPACITY
CARDIAC-FUNCTION
Mitochondrial biogenesis
Physical Conditioning, Animal
Physiology (medical)
Internal medicine
Mitophagy
Receptors, Erythropoietin
medicine
Animals
Myocyte
Cardiomegaly, Exercise-Induced
Muscle, Skeletal
Mice, Knockout
Organelle Biogenesis
business.industry
Myocardium
food and beverages
Skeletal muscle
RECOMBINANT-HUMAN-ERYTHROPOIETIN
Exercise performance
Cardiac and skeletal muscle
Adaptation, Physiological
ISCHEMIA
MICE
DIFFERENTIATION
Endocrinology
medicine.anatomical_structure
Erythropoietin
VOLUME
embryonic structures
HEART-FAILURE
Erythropoiesis
business
medicine.drug
Subjects
Details
- ISSN :
- 14322013 and 00316768
- Volume :
- 473
- Database :
- OpenAIRE
- Journal :
- Pflügers Archiv - European Journal of Physiology
- Accession number :
- edsair.doi.dedup.....371e6f2e3e5fb3b86f7a3650108578b2