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Spalt-Like Protein 4 (SALL4) Promotes Angiogenesis by Activating Vascular Endothelial Growth Factor A (VEGFA) Signaling
- Source :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Publication Year :
- 2020
-
Abstract
- BACKGROUND Spalt-like protein 4 (SALL4) is a nuclear transcription factor central to early embryonic development, especially for regulating pluripotency of embryonic stem cells (ESCs) and sustaining ESCs self-renewal. Aberrant re-expression of SALL4 in adult tissues is involved in tumorigenesis and cancer progression. However, the role of SALL4 in angiogenesis remains elusive. Here, we determined the potential action of SALL4 on proliferation, migration, and tube formation of endothelial cells. MATERIAL AND METHODS HUVECs were infected with lentiviral particles expressing shRNA against SALL4. QRT-PCR and immunoblotting analysis were carried out to evaluate knockdown efficiency at mRNA and protein levels. Cell proliferation was measured by CCK-8 assay and flow cytometry was conducted to analyze cell cycle distribution. Wound-healing and Transwell migration assays were performed to evaluate cell motility. In addition, we determined the role of SALL4 on angiogenesis by tube formation assay, and Western blot analysis was used to assess the effect of SALL4 downregulation on VEGFA expression. RESULTS We found that SALL4 downregulation resulted in decreased proliferation. Cell cycle analysis revealed that SALL4 knockdown impeded cell cycle progression and induced cell cycle arrest at G1 phase. We also found that silencing of SALL4 decreased the capacity of wound healing and cell migration in HUVECs. Furthermore, tube formation assay showed that loss of SALL4 inhibited HUVECs angiogenesis. We also observed that SALL4 knockdown reduced the level of VEGFA in HUVECs. CONCLUSIONS In conclusion, these results support that by promoting proliferation, cell cycle progression, migration, and tube formation, SALL4 is involved in the process of angiogenesis through modulating VEGFA expression.
- Subjects :
- Vascular Endothelial Growth Factor A
Cell cycle checkpoint
Angiogenesis
Neovascularization, Physiologic
030204 cardiovascular system & hematology
03 medical and health sciences
0302 clinical medicine
Cell Movement
Lab/In Vitro Research
Human Umbilical Vein Endothelial Cells
Humans
Angiogenic Proteins
Cell Proliferation
Tube formation
Gene knockdown
Cell growth
Chemistry
G1 Phase
Endothelial Cells
Cell migration
General Medicine
Cell cycle
eye diseases
Cell biology
Vascular endothelial growth factor A
Gene Expression Regulation
030220 oncology & carcinogenesis
Angiogenesis Inducing Agents
Cell Migration Assays
Signal Transduction
Transcription Factors
Subjects
Details
- ISSN :
- 16433750
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Medical science monitor : international medical journal of experimental and clinical research
- Accession number :
- edsair.doi.dedup.....37443d34272cb4f8e061787636e15e7e