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Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker
- Source :
- PLoS ONE, Vol 11, Iss 7, p e0160031 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- For rapid screening and quantification of an antisera antibody, a nanometer bithiophene-based conductive biolinker can enhanced signal performance and can be used to verify the interaction of an anti-IFN-γ antibody with an IFN-γ protein. The experimental measurements take a generic approach which takes advantage of the functionality of thiophene-based linkers for biosensors. Effects associated with using bithiophene as a biolinker for surface plasmon resonance (SPR) spectroscopy are examined in this paper. By using an atomic force microscope (AFM), it was observed that the morphology of the bithiophene modified gold sensor surface became smoother than the original gold surface. We compared the response and concentration of the anti-IFN-γ antibody on a bithiophene-coated and dextran-coated biochip as well as on different thickness-modified surfaces under SPR relevant conditions. The results indicate that a response to IFN-γ molecules immobilized on a sensor using a bithiophene biolinker improved more than 8-fold when compared to that of a sensor using a dextran biolinker. Furthermore, the regeneration ability of the sensor surface shows good repeatability as only less than a 1% decrease was found after repeating the experimental work over 6 cycles. The characteristics provided us with a good platform for rapid screening, real-time monitoring and quantitative concentration of the autoimmune antibody activities.
- Subjects :
- 0301 basic medicine
Glycobiology
Antibody Response
lcsh:Medicine
Reflection
02 engineering and technology
Biosensing Techniques
Biochemistry
chemistry.chemical_compound
Mice
Thiophene
Medicine and Health Sciences
Nanotechnology
Surface plasmon resonance
Enzyme-Linked Immunoassays
Biochip
lcsh:Science
Glucans
Immune Response
Microscopy
Multidisciplinary
Physics
Classical Mechanics
Dextrans
Detectors
Repeatability
021001 nanoscience & nanotechnology
Precipitation Techniques
Atomic Force Microscopy
Chemistry
Dextran
Physical Sciences
Engineering and Technology
0210 nano-technology
Research Article
Chemical Elements
Materials science
Immunology
Equipment
Thiophenes
Research and Analysis Methods
Sensitivity and Specificity
Antibodies
03 medical and health sciences
Interferon-gamma
Polysaccharides
Animals
Humans
Immunoprecipitation
Spectroscopy
Immunoassays
Scanning Probe Microscopy
lcsh:R
Biology and Life Sciences
Molecular biology
030104 developmental biology
Immobilized Proteins
Biosensors
chemistry
Biophysics
Immunologic Techniques
Nanometre
lcsh:Q
Gold
Biosensor
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3748609e8adf1c3b2e6c579f6e63be93