Back to Search
Start Over
Free RCK Arrangement in Kch, a Putative Escherichia coli Potassium Channel, as Suggested by Electron Crystallography
- Source :
- Structure. 23:199-205
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- SummaryThe ligand-gated potassium channels are stimulated by various kinds of messengers. Previous studies showed that ligand-gated potassium channels containing RCK domains (the regulator of the conductance of potassium ion) form a dimer of tetramer structure through the RCK octameric gating ring in the presence of detergent. Here, we have analyzed the structure of Kch, a channel of this type from Escherichia coli, in a lipid environment using electron crystallography. By combining information from the 3D map of the transmembrane part of the protein and docking of an atomic model of a potassium channel, we conclude that the RCK domains face the solution and that an RCK octameric gating ring arrangement does not form under our crystallization condition. Our findings may be applied to other potassium channels that have an RCK gating ring arrangement.
- Subjects :
- Models, Molecular
Potassium Channels
Potassium
chemistry.chemical_element
Gating
Crystallography, X-Ray
Protein structure
Tetramer
Structural Biology
Protein Interaction Maps
Protein Structure, Quaternary
Molecular Biology
Crystallography
Chemistry
Electron crystallography
Escherichia coli Proteins
Lipids
Transmembrane protein
Potassium channel
Protein Structure, Tertiary
Molecular Docking Simulation
Microscopy, Electron
Structural biology
Biophysics
Ion Channel Gating
Subjects
Details
- ISSN :
- 09692126
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Structure
- Accession number :
- edsair.doi.dedup.....3749727e9f41c7da4c075d139667a8bd