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Toxicity of food contact paper evaluated by combined biological and chemical methods
- Source :
- Toxicology in vitro : an international journal published in association with BIBRA. 59
- Publication Year :
- 2018
-
Abstract
- The study was focused on assessment of potential health risks of paper-based food contact materials (FCMs) in a step-wise approach using three toxicological bioassays in vitro and chemical analyses of migrating contaminants. 3T3 NRU cytotoxicity test showed high sensitivity to detect basal toxicity of FCMs extracts and served as a first-line test for selection of samples for further testing. The reconstructed human intestine model EpiIntestinal showed more realistic tissue response than cell culture monolayer and higher resistance despite prolonged exposure to the selected 6 samples, i.e. negligible decrease of viability and intestinal penetration, nevertheless an increase of IL-8 after exposure to black printed sample extract. Yeast based assays identified weak agonistic/antagonostic activity to human androgen receptor of the black printed sample. In accordance with the biological effects, the targeted LC and GC analytical methods confirmed the presence of high amounts of phthalates, photoinitiators and PAHs that could justify the hazard of the black printed sample. Heavily printed uncoated FCMs are recognized not to be suitable for direct contact with food. The selected bioassays and chemical analyses might be useful tools to detect targeted biological effects of xenobiotics suspected to contribute to human exposure from food.
- Subjects :
- 0301 basic medicine
Paper
Cytotoxicity test
Food contact materials
BALB 3T3 Cells
Cell Survival
Phthalic Acids
Food Contamination
Saccharomyces cerevisiae
Toxicology
Risk Assessment
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Bioassay
Animals
Humans
Food science
Intestinal Mucosa
Polycyclic Aromatic Hydrocarbons
Food contact
Chemistry
Food Packaging
General Medicine
Contamination
In vitro
030104 developmental biology
Intestinal Absorption
Receptors, Estrogen
Receptors, Androgen
030220 oncology & carcinogenesis
Toxicity
Cytokines
Biological Assay
Xenobiotic
Subjects
Details
- ISSN :
- 18793177
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Accession number :
- edsair.doi.dedup.....375d4faef8640200039892702146c8fb