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Preparation of ICA-loaded mPEG-ICA nanoparticles and their application in the treatment of LPS-induced H9c2 cell damage
- Source :
- Nanoscale Research Letters, Vol 16, Iss 1, Pp 1-16 (2021), Nanoscale Research Letters
- Publication Year :
- 2021
- Publisher :
- SpringerOpen, 2021.
-
Abstract
- Hydrophilic polyethylene glycol monomethyl ether (mPEG) was grafted onto Icariin (ICA) by succinic anhydride to form a polyethylene glycol-Icariin (mPEG-ICA) polymer. The structure of the polymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). mPEG-ICA nanoparticles loaded with ICA were prepared by physical embedding of ICA by dialysis. The particle size was determined to be (220 ± 13.7) nm, and the ζ potential was (2.30 ± 1.33) mV by dynamic light scattering (DLS). Under a transmission electron microscope (TEM), the nanoparticles were spherical, and the morphology was regular. In the medium with pH 7.4, the drug release rate of mPEG-ICA nanoparticles reached (52.80 ± 1.70)% within 72 h. At pH 6.8, the cumulative drug release of nanoparticles reached (75.66 ± 0.17)% within 48 h. Treatment of the nanoparticles with LPS-treated H9c2 cells maintained cell viability, reduced LDH release and exerted antiapoptotic effects. Moreover, ICA-loaded mPEG-ICA nanoparticles significantly decreased the mRNA expression of the myocardial inflammatory cytokines TNF-α, IL-1β and IL-6M. In conclusion, ICA-loaded mPEG-ICA nanoparticles protected against LPS-induced H9c2 cell injury.
- Subjects :
- chemistry.chemical_classification
Dynamic light scattering (DLS)
Materials science
Nano Express
Fourier infrared spectroscopy
Succinic anhydride
Nanochemistry
Nanoparticle
Polymer
Nuclear magnetic resonance spectroscopy
Condensed Matter Physics
chemistry.chemical_compound
Icariin (ICA)
chemistry
Dynamic light scattering
cardiovascular system
TA401-492
General Materials Science
Particle size
Fourier transform infrared spectroscopy
Polyethylene glycol monomethyl ether (mPEG)
Nuclear magnetic resonance spectroscopy (NMR)
Materials of engineering and construction. Mechanics of materials
Nuclear chemistry
Subjects
Details
- Language :
- English
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nanoscale Research Letters
- Accession number :
- edsair.doi.dedup.....37777eabc1139db033557719f9b709b3