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Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue
- Source :
- Molecules, Vol 25, Iss 1794, p 1794 (2020), Molecules, Volume 25, Issue 8
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.
- Subjects :
- 0106 biological sciences
DPPH
Staphylococcus aureus
Pharmaceutical Science
01 natural sciences
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
Tissue culture
lcsh:Organic chemistry
Drug Discovery
Caffeic acid
Venus flytrap
Food science
Physical and Theoretical Chemistry
030304 developmental biology
Cronobacter sakazakii
0303 health sciences
antibiotic-resistant bacteria
Phenylpropanoid
biology
Venus Flytrap
Escherichia coli
Organic Chemistry
Plumbagin
biology.organism_classification
plant secondary metabolites
Elicitor
chemistry
biotic elicitation
Chemistry (miscellaneous)
Molecular Medicine
010606 plant biology & botany
Ellagic acid
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....37819451774e5d74153454d62693517d
- Full Text :
- https://doi.org/10.3390/molecules25081794