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Signal Sequence Cleavage of Peptidyl-tRNA Prior to Release from the Ribosome and Translocon
- Source :
- Journal of Biological Chemistry. 279:24919-24922
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Many secretory polypeptides undergo cleavage of their signal sequence. In this study, we observed and quantitated the presence of a tRNA-bound, ribosome-associated polypeptide subpopulation present in vitro. This subpopulation was accessible to signal peptidase on ribosome-associated polypeptides longer than 114 amino acids. This demonstrates that it is possible for a peptidyl-tRNA species, in the midst of translation, to be processed by the endoplasmic reticulum signal peptidase implying that the peptidase is closely associated with the mammalian translocon.
- Subjects :
- Signal peptide
Signal peptidase
Signal recognition particle
Endoplasmic reticulum
Cell Biology
Protein Sorting Signals
RNA, Transfer, Amino Acyl
Biology
Endoplasmic Reticulum
Translocon
Biochemistry
Ribosome
beta-Lactamases
Molecular Weight
Protein Transport
Protein Biosynthesis
Transfer RNA
Ribosomes
Molecular Biology
Signal peptide peptidase
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....3781b000c6aea79b2582f8380b86a007