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Glycan biomarkers of autoimmunity and bile acid-associated alterations of the human glycome
- Source :
- Clinical Immunology, 230. ACADEMIC PRESS INC ELSEVIER SCIENCE, Clinical Immunology
- Publication Year :
- 2021
-
Abstract
- We have recently introduced multiple reaction monitoring (MRM) mass spectrometry as a novel tool for glycan biomarker research and discovery. Herein, we employ this technique to characterize the site-specific glycan alterations associated with primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). Glycopeptides associated with disease severity were also identified. Multinomial regression modelling was employed to construct and validate multi-analyte diagnostic models capable of accurately distinguishing PBC, PSC, and healthy controls from one another (AUC=0.93±0.03). Finally, to investigate how disease-relevant environmental factors can influence glycosylation, we characterized the ability of bile acids known to be differentially expressed in PBC to alter glycosylation. We hypothesize that this could be a mechanism by which altered self-antigens are generated and become targets for immune attack. This work demonstrates the utility of the MRM method to identify diagnostic site-specific glycan classifiers capable of distinguishing even related autoimmune diseases from one another.
- Subjects :
- Liver Cirrhosis
Glycosylation
Primary Sclerosing Cholangitis (PSC)
Cholangitis
Multiple Reaction Monitoring
Autoimmunity
medicine.disease_cause
Sclerosing
chemistry.chemical_compound
Primary biliary cirrhosis
Diagnosis
Immunology and Allergy
Medicine
Glycomics
N-glycopeptides
B-Lymphocytes
Primary Biliary Cirrhosis
Bile acid
biology
Liver Cirrhosis, Biliary
Liver Disease
Primary Biliary Cirrhosis (PBC)
Biliary
Electrospray Ionization
Glycopeptides
Multiple 
Biomarker (medicine)
Glycan
Spectrometry, Mass, Electrospray Ionization
medicine.drug_class
Cholangitis, Sclerosing
Chronic Liver Disease and Cirrhosis
Immunology
biomarker testing
Autoimmune Disease
Primary sclerosing cholangitis
Diagnosis, Differential
Bile Acids and Salts
Rare Diseases
Polysaccharides
Clinical Research
Primary Sclerosing Cholangitis
Humans
Reacton Monitoring
Digestive Diseases - (Gallbladder)
plasma
Multiple Reacton Monitoring
business.industry
Spectrometry
Mass
medicine.disease
Glycome
chemistry
Case-Control Studies
Differential
biology.protein
business
Digestive Diseases
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Clinical Immunology, 230. ACADEMIC PRESS INC ELSEVIER SCIENCE, Clinical Immunology
- Accession number :
- edsair.doi.dedup.....37c26e3c65f7159328b27e819b68cf58