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Biochemical and Pharmacological Aspects of Two Bradykinin-Potentiating Peptides Obtained from Tryptic Hydrolysis of Casein
- Source :
- Journal of Protein Chemistry. 22:601-606
- Publication Year :
- 2003
- Publisher :
- Springer Science and Business Media LLC, 2003.
-
Abstract
- Peptides that display bradykinin-potentiating activity have been obtained from a number of distinct sources, such as snake venoms, fibrinogen, and casein. This paper describes the characterization of two new peptides generated by tryptic hydrolysis of casein. No homology was found with other known vasoactive or vasopotentiating peptides, especially by the lack of Ile-Pro-Pro motif. The peptides EMPFPK and YPVEPFTE, corresponding to the gamma casein sequence (108-113 and 114-121, respectively), displayed a selective potentiating activity on isolated guinea pig ileum for bradykinin. Besides, the octapeptide YPVEPFTE showed an in vitro competitive inhibitor effect on angiotensin-converting enzyme and thimet oligopeptidase and presented an opiate-like activity, increasing two times the latence time in the hot-plate assay. The results suggest that the isolated bioactive peptides act on conversion and/or inactivation of endogenous peptides by enzymes such as angiotensin-converting enzyme and thimet oligopeptidase by modifying several systemic responses such as blood-pressure regulation and in pain response.
- Subjects :
- Guinea Pigs
Pain
Bradykinin
Angiotensin-Converting Enzyme Inhibitors
Blood Pressure
Endogeny
In Vitro Techniques
Biochemistry
chemistry.chemical_compound
Ileum
Casein
Animals
Bioorganic chemistry
Trypsin
Amino Acid Sequence
chemistry.chemical_classification
Thimet oligopeptidase
biology
Hydrolysis
Uterus
Caseins
Metalloendopeptidases
Angiotensin-converting enzyme
Molecular biology
In vitro
Rats
Enzyme
chemistry
biology.protein
Female
Oligopeptides
Subjects
Details
- ISSN :
- 15734943 and 02778033
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Journal of Protein Chemistry
- Accession number :
- edsair.doi.dedup.....37e06aee7538ef9a547d68905893c86f