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Increased in-stent stenosis in ApoE knockout mice: insights from a novel mouse model of balloon angioplasty and stenting
- Source :
- Europe PubMed Central
- Publication Year :
- 2016
-
Abstract
- Objective— We aimed to develop and validate a model of angioplasty and stenting in mice that would allow investigation of the response to stent injury using genetically modified mouse strains. Methods and Results— Aortic segments from either C57BL/6 wild-type or atherosclerotic ApoE-KO mice underwent balloon angioplasty alone or balloon angioplasty and stenting with a 1.25×2.5 mm stainless steel stent. Vessels were carotid-interposition grafted into genetically identical littermate recipients and harvested at 1, 7, 14, or 28 days. In wild-type mice, stenting generated an inflammatory vascular injury response between days 1 to 7, leading to the development of neointimal hyperplasia by day 14, which further increased in area by day 28 leading to the development of in-stent stenosis. Uninjured vessels and vessels injured by balloon angioplasty alone developed minimal neointimal hyperplasia. In stented ApoE-KO mice, neointimal area at 28 days was 30% greater compared with wild-type mice. Conclusions— By reproducing important features of human stenting in atherosclerotic mice, we provide the potential to investigate molecular pathways and evaluate novel therapeutic targets for stent injury and restenosis.
- Subjects :
- Male
Vasculitis
medicine.medical_specialty
Time Factors
medicine.medical_treatment
Aorta, Thoracic
Constriction, Pathologic
Balloon
Mice
Apolipoproteins E
Restenosis
Internal medicine
Angioplasty
Medicine
Animals
cardiovascular diseases
Neointimal hyperplasia
Mice, Knockout
Hyperplasia
business.industry
Vascular disease
Immunochemistry
Stent
medicine.disease
Atherosclerosis
equipment and supplies
Surgery
Mice, Inbred C57BL
Stenosis
Carotid Arteries
surgical procedures, operative
Cardiology
Female
Stents
Cardiology and Cardiovascular Medicine
business
Tunica Intima
Angioplasty, Balloon
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....37e1281c6700c25be1b9f9a7b87166fc