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Epigallocatechin-3-Gallate-Loaded Liposomes Favor Anti-Inflammation of Microglia Cells and Promote Neuroprotection
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 3037, p 3037 (2021), Volume 22, Issue 6
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Microglia-mediated neuroinflammation is recognized to mainly contribute to the progression of neurodegenerative diseases. Epigallocatechin-3-gallate (EGCG), known as a natural antioxidant in green tea, can inhibit microglia-mediated inflammation and protect neurons but has disadvantages such as high instability and low bioavailability. We developed an EGCG liposomal formulation to improve its bioavailability and evaluated the neuroprotective activity in in vitro and in vivo neuroinflammation models. EGCG-loaded liposomes have been prepared from phosphatidylcholine (PC) or phosphatidylserine (PS) coated with or without vitamin E (VE) by hydration and membrane extrusion method. The anti-inflammatory effect has been evaluated against lipopolysaccharide (LPS)-induced BV-2 microglial cells activation and the inflammation in the substantia nigra of Sprague Dawley rats. In the cellular inflammation model, murine BV-2 microglial cells changed their morphology from normal spheroid to activated spindle shape after 24 h of induction of LPS. In the in vitro free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, EGCG scavenged 80% of DPPH within 3 min. EGCG-loaded liposomes could be phagocytized by BV-2 cells after 1 h of cell culture from cell uptake experiments. EGCG-loaded liposomes improved the production of BV-2 microglia-derived nitric oxide and TNF-α following LPS. In the in vivo Parkinsonian syndrome rat model, simultaneous intra-nigral injection of EGCG-loaded liposomes attenuated LPS-induced pro-inflammatory cytokines and restored motor impairment. We demonstrated that EGCG-loaded liposomes exert a neuroprotective effect by modulating microglia activation. EGCG extracted from green tea and loaded liposomes could be a valuable candidate for disease-modifying therapy for Parkinson’s disease (PD).
- Subjects :
- Lipopolysaccharides
phosphatidylserine
Cell Survival
Anti-Inflammatory Agents
Inflammation
Pharmacology
Nitric Oxide
complex mixtures
Neuroprotection
Article
Catechin
Catalysis
neuroinflammation
Cell Line
lcsh:Chemistry
L-α-phosphatidylcholine
Inorganic Chemistry
Mice
chemistry.chemical_compound
In vivo
medicine
Animals
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Cell Shape
Molecular Biology
Spectroscopy
Neuroinflammation
Liposome
Behavior, Animal
Microglia
Organic Chemistry
food and beverages
General Medicine
Phosphatidylserine
Computer Science Applications
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Cell culture
Liposomes
Parkinson’s disease
Cytokines
sense organs
medicine.symptom
Biomarkers
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....380b627f7e3f515a8863bf3e4e7d0054
- Full Text :
- https://doi.org/10.3390/ijms22063037