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Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port, Shanghai, China
- Source :
- Marine pollution bulletin. 160
- Publication Year :
- 2020
-
Abstract
- Ballast water is a major vector of invasion by protozoans and metazoans. Bacterial invasion is less-well understood. We surveyed the bacterial diversity of ballast water from 26 container ships arriving at the Yangshan Deepwater Port, Shanghai, China during 2015-2016. We characterized the ballast microbiome using high-throughput sequencing (HTS) based on V4-V5 region of 16S rRNA genes. We simultaneously monitored physicochemical parameters of the ballast water, including temperature, pH, dissolved oxygen (DO), salinity, turbidity, total suspended solid (TSS), particulate organic carbon (POC), NO2, NH4, PO4. Proteobacteria was the dominant phylum, comprising more than 50% of the OTUs of almost all vessels, followed by Bacteroidetes (12.08%), Actinobacteria (4.86%) Planctomycetes (3.24%) and Cyanobacteria (1.95%). The relative abundance of Cyanobacteria differed among vessels. It was negatively correlated with temperature, NO3, pH, TSS, PO4, and turbidity and positively correlated with NH4, POC. The genus Synechococcus was the most common Cyanobacteria in our results. Escherichia coli were relatively rare; they are indicator-species of D-2 standards published by the IMO. The relative abundance of the genus Vibrio ranged from 0.003% to 24.88% among different vessels. Our results showed that HTS was able to profile the bacterial communities in ballast-waters, even when the approach was restricted by technical and other obstacles.
- Subjects :
- 0106 biological sciences
Ballast
Cyanobacteria
China
biology
010604 marine biology & hydrobiology
Microbiota
Planctomycetes
Bacteroidetes
010501 environmental sciences
Aquatic Science
Oceanography
biology.organism_classification
01 natural sciences
Pollution
Actinobacteria
Salinity
Environmental chemistry
RNA, Ribosomal, 16S
Environmental science
Proteobacteria
Turbidity
Ships
0105 earth and related environmental sciences
Vibrio
Subjects
Details
- ISSN :
- 18793363
- Volume :
- 160
- Database :
- OpenAIRE
- Journal :
- Marine pollution bulletin
- Accession number :
- edsair.doi.dedup.....38378b0aea563e5c66113dbec85e0d0c