Additional file 3 of Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor

Additional file 3. Supplementary figure 3. Arterial and vein differentiation of hPSC-ECs. (A) Differentiation scheme of hPSC-EB-CD144+ into hPSC-ECs in the presence or absence of DAPT and representative phase-contrast microscopy images after differentiation. Scale Bar 500 μm. Flow cytometry analysis of the endothelial phenotype of hPSC-ECs in the presence and absence of DAPT. (B) Confocal immunofluorescent image of Matrigel networks formed by hPSC-ECs. CD31 in green and DAPI in blue. Scale Bar: 100 μm.