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Zebrafish prmt3 negatively regulates antiviral responses
- Source :
- The FASEB Journal. 34:10212-10227
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Arginine methylation catalyzed by protein arginine methyltransferases (PRMT) is a common post-translational modification in histone and nonhistone proteins, which regulates many cellular functions. Protein arginine methyltransferase 3 (prmt3), a type I arginine methyltransferase, has been shown to carry out the formation of stable monomethylarginine as an intermediate before the establishment of asymmetric dimethylarginine. To date, however, the role of PRMT3 in antiviral innate immunity has not been elucidated. This study showed that zebrafish prmt3 was upregulated by virus infection and that the overexpression of prmt3 suppressed cellular antiviral response. The PRMT3 inhibitor, SGC707, enhanced antiviral capability. Consistently, prmt3-null zebrafish were more resistant to Spring Viremia of Carp Virus (SVCV) and Grass Carp Reovirus (GCRV) infection. Further assays showed that the overexpression of prmt3 diminished the phosphorylation of irf3 and prmt3 interacted with rig-i. In addition, both zinc-finger domain and catalytic domain of prmt3 were required for the suppressive function of prmt3 on IFN activation. Our findings suggested that zebrafish prmt3 negatively regulated the antiviral responses, implicating the vital role of prmt3-or even arginine methylation-in antiviral innate immunity.
- Subjects :
- 0301 basic medicine
Protein-Arginine N-Methyltransferases
Methyltransferase
Arginine
Biology
Antiviral Agents
Methylation
Biochemistry
Histones
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Rhabdoviridae Infections
Genetics
Animals
Phosphorylation
Molecular Biology
Zebrafish
Cells, Cultured
Innate immune system
Zinc Fingers
Isoquinolines
biology.organism_classification
Immunity, Innate
Up-Regulation
Cell biology
030104 developmental biology
Virus Diseases
Rhabdoviridae
IRF3
Protein Processing, Post-Translational
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....3842bcc1f77fbb27ff2abc371621a5a8