Back to Search
Start Over
Bisphenol F promotes the secretion of pro-inflammatory cytokines in macrophages by enhanced glycolysis through PI3K-AKT signaling pathway
- Source :
- Toxicology Letters. 350:30-39
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Bisphenol F (BPF) is a member of endocrine disrupting chemicals (EDCs). As a substitute of bisphenol A (BPA), BPF is widely used in various consumer products, leading to an increased risk of people's exposure. However, there are few studies on the immunotoxicity and mechanism of BPF. This study aimed to investigate the effect of BPF on the secretion of pro-inflammatory cytokines by macrophages and explore its mechanism. In our study, RAW264.7 macrophages were treated with different concentrations of BPF (0, 5, 10 and 20 μM) for 24 h. The results showed that the secretion of pro-inflammatory cytokines (IL-6, TNF-α and IL-1β) and the production of lactate were increased in a dose-dependent manner. BPFalso led to the activation of the PI3K-AKT signaling pathway. After pretreatment with glycolysis inhibitor (2-DG) and exposure to BPF (20 μM), the secretion of pro-inflammatory cytokines induced by BPF was inhibited. PI3K inhibitor (LY294002) and estrogen receptor (ER) antagonist (ICI 182,780) could also inhibit the above effects induced by BPF (20 μM). In conclusion, our results suggested that BPF can enhance glycolysis through ER mediated PI3K-AKT signaling pathway, and the enhanced glycolysis further promoted the secretion of pro-inflammatory cytokines. Our research provides basic data for future studies on bisphenol exposure and immunotoxicity.
- Subjects :
- 0301 basic medicine
Bisphenol
Inflammation
Pharmacology
Toxicology
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phenols
medicine
Humans
Glycolysis
LY294002
Secretion
Benzhydryl Compounds
Cells, Cultured
PI3K/AKT/mTOR pathway
Immunotoxins
Macrophages
General Medicine
030104 developmental biology
chemistry
Cytokines
medicine.symptom
Signal transduction
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 03784274
- Volume :
- 350
- Database :
- OpenAIRE
- Journal :
- Toxicology Letters
- Accession number :
- edsair.doi.dedup.....38454f51d92aef4d083bef6d89ad6bb2