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Detection of bcrabl fusion mRNA in samples of whole, unfractionated blood
- Source :
- Leukemia research. 18(6)
- Publication Year :
- 1994
-
Abstract
- Using a new strategy, capture-RT-PCR, bcrabl fusion mRNA sequences were specifically and sensitively detected in samples of whole blood from leukemia patients with the Philadelphia chromosome. Sample processing required only mixing blood with the chaotropic salt, GuSCN, and mRNA was captured during a short incubation of prepared blood with an affinity membrane. Immobilized mRNA sequences were amplified without elution. No radioisotopes or Southern transfer were needed.
- Subjects :
- Cancer Research
Messenger RNA
Blood Specimen Collection
Leukemia
Fusion Proteins, bcr-abl
Hematology
Biology
Nucleotidyltransferase
medicine.disease
Philadelphia chromosome
Molecular biology
Polymerase Chain Reaction
law.invention
Chaotropic agent
Kinetics
Oncology
law
medicine
Humans
RNA, Messenger
Incubation
Polymerase chain reaction
Whole blood
Subjects
Details
- ISSN :
- 01452126
- Volume :
- 18
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Leukemia research
- Accession number :
- edsair.doi.dedup.....3862640d716a44333867706513ace822