Back to Search
Start Over
Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus
- Source :
- Vaccine. 24(11)
- Publication Year :
- 2004
-
Abstract
- A human influenza A virus X-31 (high-yielding strain) was cold-adapted for possible future use as live attenuated vaccine. Mutant influenza viruses were selected during successive serial passage in embryonated hens' eggs at progressively lower sub-optimal temperature (30, 27 degrees C followed by 24 degrees C). The cold-passaged mutant exhibited both temperature-sensitivity (ts) and cold-adapted (ca) phenotypes. The pathogenicity and immunogenicity of X-31 ca virus were studied in mice following intranasal inoculation. The mice did not show clinical signs even at high titer infection. Immunization of mice with X-31 ca virus elicited high titers of neutralizing antibody and provided complete protection against homologous and heterologous virus challenges. To assess the genetic stability, the X-31 ca virus was passaged at 37 degrees C in MDCK cells or inoculated into mice. Revertant virus was not found in the lungs of any of the mice and the supernatants of the MDCK culture. We conclude that the X-31 ca candidate vaccine virus exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for live attenuated vaccine and merits further evaluation at clinical level.
- Subjects :
- Influenza vaccine
Orthomyxoviridae
Enzyme-Linked Immunosorbent Assay
Chick Embryo
Viral Plaque Assay
Biology
medicine.disease_cause
Antibodies, Viral
Vaccines, Attenuated
Virus
Microbiology
Cell Line
Influenza A Virus, H2N2 Subtype
Mice
Dogs
Orthomyxoviridae Infections
Serial passage
Neutralization Tests
Influenza A virus
medicine
Animals
Lung
Administration, Intranasal
Mice, Inbred BALB C
Attenuated vaccine
General Veterinary
General Immunology and Microbiology
Immunogenicity
Influenza A Virus, H3N2 Subtype
Body Weight
Public Health, Environmental and Occupational Health
biology.organism_classification
Virology
Adaptation, Physiological
Cold Temperature
Titer
Infectious Diseases
Phenotype
Influenza Vaccines
Molecular Medicine
Female
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 24
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....3869fa88f6be5e5a62d8eeb123a0d458