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Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus

Authors :
Jae Min Song
Sang Uk Seo
Kwang Hee Lee
Chung Min Lee
Hyun Ah Kim
Baik Lin Seong
Source :
Vaccine. 24(11)
Publication Year :
2004

Abstract

A human influenza A virus X-31 (high-yielding strain) was cold-adapted for possible future use as live attenuated vaccine. Mutant influenza viruses were selected during successive serial passage in embryonated hens' eggs at progressively lower sub-optimal temperature (30, 27 degrees C followed by 24 degrees C). The cold-passaged mutant exhibited both temperature-sensitivity (ts) and cold-adapted (ca) phenotypes. The pathogenicity and immunogenicity of X-31 ca virus were studied in mice following intranasal inoculation. The mice did not show clinical signs even at high titer infection. Immunization of mice with X-31 ca virus elicited high titers of neutralizing antibody and provided complete protection against homologous and heterologous virus challenges. To assess the genetic stability, the X-31 ca virus was passaged at 37 degrees C in MDCK cells or inoculated into mice. Revertant virus was not found in the lungs of any of the mice and the supernatants of the MDCK culture. We conclude that the X-31 ca candidate vaccine virus exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for live attenuated vaccine and merits further evaluation at clinical level.

Details

ISSN :
0264410X
Volume :
24
Issue :
11
Database :
OpenAIRE
Journal :
Vaccine
Accession number :
edsair.doi.dedup.....3869fa88f6be5e5a62d8eeb123a0d458