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Elevated miR-34c-5p Mediates Dermal Fibroblast Senescence by Ultraviolet Irradiation
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2013
- Publisher :
- Ivyspring International Publisher, 2013.
-
Abstract
- Previous studies showed that several miRNAs can regulate pathways involved in UVB-induced premature senescence and response to ultraviolet irradiation. It has also been reported that miR-34c-5p may be involved in senescence-related mechanisms. We propose that miR-34c-5p may play a crucial role in senescence of normal human primary dermal fibroblasts. Here, we explored the roles of miR-34c-5p in UVB-induced premature senescence on dermal fibroblasts. MiR-34c-5p expression was increased in dermal fibroblasts after repeated subcytotoxic UVB treatments. Underexpression of miR-34c-5p in dermal fibroblasts led to a marked delay of many senescent phenotypes induced by repeated UVB treatments. Furthermore, underexpression of miR-34c-5p in dermal fibroblasts can antagonize the alteration of G1-arrested fibroblasts. Moreover, E2F3, which can inactivate p53 pathway and play a role in cell cycle progression, is a down-stream target of miR-34c-5p. Forced down-expression of miR-34c-5p decreased the expression of UVB-SIPS induced P21 and P53 at both mRNA and protein levels. Our data demonstrated that down-regulation of miR-34c-5p can protect human primary dermal fibroblasts from UVB-induced premature senescence via regulations of some senescence-related molecules.
- Subjects :
- Male
Senescence
Ultraviolet Rays
Down-Regulation
Biology
Applied Microbiology and Biotechnology
Dermal fibroblast
Downregulation and upregulation
microRNA
Humans
miR-34c-5p
Child
p21-activated kinases
Molecular Biology
Cells, Cultured
Cellular Senescence
Ecology, Evolution, Behavior and Systematics
Skin
Messenger RNA
integumentary system
Cell Biology
Fibroblasts
Phenotype
Cell biology
MicroRNAs
premature senescence
p21-Activated Kinases
E2F3 Transcription Factor
human skin fibroblasts
Tumor Suppressor Protein p53
UVB
Cell aging
Research Paper
Developmental Biology
Subjects
Details
- ISSN :
- 14492288
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....3888af0339c3f557cb4f8380d4c6e8f8