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Comparative transcriptome analysis provides insights into the molecular mechanism underlying double fertilization between self-crossed Solanum melongena and that hybridized with Solanum aethiopicum
- Source :
- PLoS ONE, PLoS ONE, Vol 15, Iss 8, p e0235962 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum melongena) breeding, as well as for broadening its genetic base. However, interspecific hybridization with wild relatives has been barely used in eggplant breeding programs, and reproductive barriers have resulted in reduced seed numbers in interspecific combinations. The mechanism underlying this phenomenon remains unclear. We hybridized females of cultivated eggplant 177 (Solanum melongena) with males of wild relatives 53 and Y11 (Solanum aethiopicum). Self-crossed 177 was the control. The seed number per control fruit was significantly higher than that of the hybrids. Paraffin sections showed no significant difference between control and 177×53 and 177×Y11. Double fertilization began 4 days post-pollination. Sperm cells were fused with egg cells 6 days post-pollination. To understand the differences in molecular mechanisms underlying this process, transcriptomes of ovaries at 0, 4, and 6 days after self-crossing and hybridization were analyzed. We screened 22,311 differentially expressed genes (DEGs) between the control and hybrids 4 and 6 days post-pollination. A total of 497 DEGs were shared among all pollination combinations. These DEGs were enriched in plant hormone transduction, cell senescence, metabolism, and biosynthesis pathways. DEG clustering analysis indicated distinct expression patterns between the control and hybrids but not between the hybrids. The DEGs in hybrids involved secondary metabolic process, phenylpropanoid metabolic process, and carboxypeptidase activity, while those in the control involved xyloglucan metabolic process, auxin-activated signaling pathway, cell wall polysaccharide metabolic process, and xyloglucosyl transferase activity. Additionally, 1683 transcription factors, including members of the AP2-ERF, MYB, bHLH, and B3 families may play important roles in self-crossing and hybridization. Our results provide insights into the regulatory mechanisms underlying variations between ovaries of self-crossed and hybrid eggplants and a basis for future studies on crossbreeding Solanum and genetic mechanisms underlying double fertilization.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Gene Expression
Plant Science
Biochemistry
01 natural sciences
Transcriptome
Carboxypeptidase activity
Gene Expression Regulation, Plant
Secondary metabolic process
Pollination
Genetics
Multidisciplinary
Plant Anatomy
Eukaryota
food and beverages
Xyloglucan metabolic process
Plants
Phenylpropanoid metabolic process
Plant Physiology
Seeds
Medicine
Metabolic Pathways
Research Article
Evolutionary Processes
Science
Biology
Solanum
03 medical and health sciences
DNA-binding proteins
Gene Regulation
Hybridization
Hybrid
Evolutionary Biology
Gene Expression Profiling
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
Regulatory Proteins
Plant Breeding
Metabolism
030104 developmental biology
Fertilization
Hybridization, Genetic
Cell wall polysaccharide metabolic process
Transcription Factors
Developmental Biology
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....38c2ebef20b1de0f820c371dc5253718