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Short communication: Pheromonicin-SA affects mRNA expression of toll-like receptors, cytokines, and lactoferrin by Staphylococcus aureus-infected bovine mammary epithelial cells
- Source :
- Journal of dairy science. 95(2)
- Publication Year :
- 2011
-
Abstract
- Pheromonicin-SA (Ph-SA) is a newly developed, engineered multidomain peptide that has a bactericidal effect against Staphylococcus aureus. The objective of this study was to characterize innate immune responses by Staph. aureus-stimulated bovine mammary epithelial cells (BMEC) following treatment with Ph-SA. Primary BMEC from one lactating Holstein cow were isolated and exposed to Staph. aureus for 2 h, and then treated with rifampicin or Ph-SA. Total RNA was isolated from BMEC at 0, 2, 6, 12, and 24 h postinfection, and the mRNA expression of selected genes, including toll-like receptor (TLR)2 and TLR4, IL-1β, IL-6, IL-8, tumor necrosis factor α (TNF-α), and lactoferrin, was quantified by real-time PCR. In the rifampicin group, increases in the expression of mRNA for TNF-α, IL-1β, IL-6, IL-8, and lactoferrin were observed at 6 h postinfection and in the expression of mRNA for TLR2 but not for TLR4 at 12 h postinfection. In the Ph-SA group, increases in the mRNA expression of TLR2, TNF-α, IL-1β, IL-6, IL-8, and lactoferrin were observed at 6 h postinfection, and an increase in TLR4 mRNA expression was observed at 24 h postinfection. At 24 h postinfection, the mRNA expression of TLR4, TNF-α, IL-1β, IL-6, IL-8, and lactoferrin was higher in the Ph-SA group than in the rifampicin group. In conclusion, Ph-SA might promote the expression of mRNA for TLR2, TLR4, the pro-inflammatory cytokines IL-1, IL-6, and TNF-α, the chemotactic factor IL-8, and lactoferrin in Staph. aureus-infected BMEC. Moreover, Ph-SA may be of value as an antibiotic in promoting innate immune responses by Staph. aureus-infected bovine mammary epithelial cells.
- Subjects :
- Staphylococcus aureus
medicine.medical_treatment
Recombinant Fusion Proteins
Interleukin-1beta
Epithelium
Microbiology
Mammary Glands, Animal
Genetics
medicine
Animals
RNA, Messenger
Receptor
Mastitis, Bovine
Messenger RNA
Toll-like receptor
Innate immune system
biology
Lactoferrin
Interleukin-6
Tumor Necrosis Factor-alpha
Interleukin-8
Toll-Like Receptors
Staphylococcal Infections
Toll-Like Receptor 2
Anti-Bacterial Agents
Toll-Like Receptor 4
TLR2
Cytokine
biology.protein
TLR4
Cytokines
Animal Science and Zoology
Cattle
Female
Food Science
Subjects
Details
- ISSN :
- 15253198
- Volume :
- 95
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of dairy science
- Accession number :
- edsair.doi.dedup.....391f6ae899e6e6c22fa6333704d5131c