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MicroRNA-223 and microRNA-21 in peripheral blood B cells associated with progression of primary biliary cholangitis patients
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 9, p e0184292 (2017)
- Publication Year :
- 2017
-
Abstract
- Recently, there is ample evidence suggesting the important role of microRNAs (miRNAs) in autoimmune diseases via modulating B cells function. Primary biliary cholangitis (PBC) is a progressive immune-mediated liver disease with unclear pathogenic mechanism. Whether the miRNA in peripheral B cells of PBC involve the mechanisms of pathology and progression is not known. The present study explores miRNA deregulation in peripheral B-cell of PBC from stage I to IV and healthy controls. Peripheral B cells were obtained from 72 PBC patients (stage I, n = 17; stage II, n = 23; stage III, n = 16; stage IV, n = 16) and 15 healthy controls. Initially, in a discovery study, miRNA array analysis was performed, subsequently, in a validation study, quantitative PCR was used to investigate miRNA expression profile in B cells of PBS patients compared to healthy controls. Based on bioinformatics analysis, we identified the potential biological processes and significant signaling pathways affected by these microRNAs, and generated the microRNA-gene network. The discovery study identified 558 miRNAs differentially expressed in B cells of PBC patients compared to controls. Interestingly, among all differentially expressed miRNAs, hsa-miR-223-3p and hsa-miR-21-5p were the only miRNAs that showed consistent and significant down-regulation from stage I to stage III of PBC. Bioinformatics showed that potential target genes of both miRNAs involved in migration, cell differentiation, apoptosis, and signal transduction pathways. In conclusion, our results suggest that the expression profiles of miRNA in peripheral B cells of PBC patients are closely associated with the disease progression, especially the down-regulation of hsa-miR-223-3p and hsa-miR-21-5p. Taken together, our study offers novel perspectives on the role of miRNAs in PBC pathogenesis.
- Subjects :
- 0301 basic medicine
Cell signaling
B Cells
Cholangitis
Cellular differentiation
lcsh:Medicine
Pathogenesis
Signal transduction
Pathology and Laboratory Medicine
Biochemistry
Transcriptome
White Blood Cells
0302 clinical medicine
Animal Cells
Gene expression
Medicine and Health Sciences
Gene Regulatory Networks
lcsh:Science
Regulation of gene expression
B-Lymphocytes
Multidisciplinary
Liver Diseases
Signaling cascades
Middle Aged
Nucleic acids
Real-time polymerase chain reaction
Disease Progression
030211 gastroenterology & hepatology
Cellular Types
Research Article
Immune Cells
Immunology
Gastroenterology and Hepatology
Biology
Autoimmune Diseases
03 medical and health sciences
microRNA
Genetics
Humans
Non-coding RNA
Antibody-Producing Cells
Blood Cells
Biology and life sciences
Gene Expression Profiling
lcsh:R
Computational Biology
Reproducibility of Results
Cell Biology
Gene regulation
Gene expression profiling
MicroRNAs
030104 developmental biology
Gene Ontology
Gene Expression Regulation
TGF-beta signaling cascade
Case-Control Studies
Cancer research
RNA
lcsh:Q
Clinical Immunology
Clinical Medicine
Biomarkers
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....3930bf0c18801421071e41efce32a9f2