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Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella
- Source :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP, Web of Science, Repositório Institucional da UNITAU, Universidade de Taubaté (UNITAU), instacron:UNITAU
- Publication Year :
- 2017
-
Abstract
- Made available in DSpace on 2019-09-12T16:53:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2017 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C albicans (CA). Tumor necrosis factor (TNF)-alpha, interleukin IL-beta, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR. (C) 2017 Elsevier Ltd. All rights reserved. [de Oliveira, Felipe Eduardo; Rossoni, Rodnei Dennis; de Barros, Patricia Pimentel; Begnini, Barbara Evelyn; Junqueira, Juliana Campos; Cardoso Jorge, Antonio Olavo; de Oliveira, Luciane Dias] Sao Paulo State Univ Unesp, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Paulo, Brazil [Pereira Leao, Mariella Vieira] Universidade de Taubaté (Unitau), Sch Med, Inst Basic Biosci, Av Tiradentes 500, BR-12030180 Sao Paulo, Brazil
- Subjects :
- 0301 basic medicine
Hemocytes
Epidemiology
Colony Count, Microbial
Probiotic
Mice
Lactobacillus acidophilus
Lactobacillus
Yeasts
Lactic acid bacteria
Innate
Candida
biology
Lacticaseibacillus rhamnosus
Candidiasis
food and beverages
Lacticaseibacillus paracasei
Corpus albicans
Galleria mellonella
Lepidoptera
Infectious Diseases
Cytokines
Infection
Lactobacillus paracasei
Cell Survival
030106 microbiology
Immunology
Mycology
Microbiology
Biofilm Formation
03 medical and health sciences
Lactobacillus rhamnosus
In vivo
Candida-Albicans
Animals
Pathogenicity
Viability assay
Macrophages
biology.organism_classification
Survival Analysis
Immune
Disease Models, Animal
Host-Defense
030104 developmental biology
RAW 264.7 Cells
In-Vitro
Antifungal Resistance
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP, Web of Science, Repositório Institucional da UNITAU, Universidade de Taubaté (UNITAU), instacron:UNITAU
- Accession number :
- edsair.doi.dedup.....393f6d1061cf981e9e8ff820f0861cac