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Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning
- Source :
- Journal of medical microbiology. 67(3)
- Publication Year :
- 2018
-
Abstract
- Purpose. Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. Methodology. Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa. Results. Differing distributions of taxa belonging to the phyla Firmicutes, Bacteroidetes, Fusobacteria, Actinobacteria, Proteobacteria, Synergistetes, Spirochaetae and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum Bacteroidetes, as well as of the species Fusobacterium nucleatum, were found in the PI group (P
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Male
Firmicutes
Gingiva
Bacterial Physiological Phenomena
Microbiology
Prevotella intermedia
03 medical and health sciences
0302 clinical medicine
RNA, Ribosomal, 16S
Tannerella forsythia
Bacteroides
Humans
Porphyromonas gingivalis
Veillonella dispar
Aged
Aged, 80 and over
biology
Bacteria
Fusobacterium nucleatum
Microbiota
Bacteroidetes
Fusobacteria
Genes, rRNA
030206 dentistry
General Medicine
Sequence Analysis, DNA
Middle Aged
biology.organism_classification
Peri-Implantitis
Bacterial Load
stomatognathic diseases
030104 developmental biology
Biofilms
Female
Synergistetes
Subjects
Details
- ISSN :
- 14735644
- Volume :
- 67
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of medical microbiology
- Accession number :
- edsair.doi.dedup.....393f8b96e8d08a1e23ea6feaf30a0515