Circular dichroism spectroscopic investigation of double-decker phthalocyanine with G-Quadruplex as promising telomerase inhibitor

Authors :: Efkan Bağda
Esra Bağda
Ebru Yabaş
[Bagda, Efkan] Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey -- [Bagda, Esra] Cumhuriyet Univ, Fac Pharm, Dept Analyt Chem, TR-58140 Sivas, Turkey -- [Yabas, Ebru] Cumhuriyet Univ, Imranh Vocat Sch, Dept Chem & Chem Proc Technol, TR-58980 Sivas, Turkey
Bagda, Efkan -- 0000-0002-3925-3430
Publication Year :: 2017
Publisher :: ELSEVIER SCIENCE BV, 2017.
Abstract: WOS: 000385901800082<br />In the present study, interaction of a double-decker phthalocyanine with two G-quadruplex DNA, Tel 21 and cMYC, was investigated. To the best of our knowledge, this is the first study about G-quadruplex-double decker phthalocyanine interaction. The spectrophotometric titration method was used for binding constant calculations. From the binding constants, it can be said that double-decker phthalocyanine more likely to bind Tel 21 rather than cMYC. The conformational changes upon binding were monitored via circular dichroism spectroscopy. The ethidium bromide replacement assay was investigated spectrofluorometrically. (C) 2016 Published by Elsevier B.V.<br />Cumhuriyet University Scientific Research Foundation Council (CUBAP) [ECZ-014]<br />This work was supported by the Cumhuriyet University Scientific Research Foundation Council (CUBAP), Project Number: ECZ-014.

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