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ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson’s and Huntington’s diseases
- Source :
- Brain Research. 1707:141-153
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- It is widely accepted that amyloid oligomers are the most toxic species to initiate the pathologic processes of Parkinson’s disease (PD) and Huntingdon’s disease (HD). But there is no definitive diagnosis for PD and HD at their early stages. Here, we conjugated an amyloid oligomer-specific scFv antibody (W20) to PEGylated superparamagnetic iron oxide nanoparticles (SPIONs) and detected the properties of the SPIONs conjugated with W20. The results showed that W20-SPIONs, with the size of around 11.8 nm in diameter, were stable and nontoxic, and had enough relaxation capacity to be used as an MRI contrast agent. When applied to the transgenic mouse models of PD and HD, W20-SPIONs crossed the blood-brain barrier and specifically bound to the oligomer area to give MRI signal, distinguishing PD and HD from healthy controls. These results indicated that W20-SPIONs had potential in early-stage diagnosis for PD and HD and also opened up a new strategy for evaluating the efficacy of new drugs.
- Subjects :
- Male
0301 basic medicine
Genetically modified mouse
Amyloid
Parkinson's disease
MRI contrast agent
Contrast Media
Mice, Transgenic
Conjugated system
Ferric Compounds
Mice
03 medical and health sciences
0302 clinical medicine
Huntington's disease
medicine
Animals
Magnetite Nanoparticles
Molecular Biology
medicine.diagnostic_test
biology
Chemistry
General Neuroscience
Parkinson Disease
Magnetic resonance imaging
medicine.disease
Magnetic Resonance Imaging
Disease Models, Animal
Huntington Disease
030104 developmental biology
Cancer research
biology.protein
Nanoparticles
Neurology (clinical)
Antibody
030217 neurology & neurosurgery
Single-Chain Antibodies
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1707
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....398885fa36ee0301eb7bfc8b399bdf8f