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Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing
- Source :
- Microchip Diagnostics, Microchip Diagnostics, pp.113-121, 2017, 978-1-4939-6732-2. ⟨10.1007/978-1-4939-6734-6_9⟩, Methods in Molecular Biology ISBN: 9781493967322
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Analyses of nucleic acids are routinely performed in hospital laboratories to detect gene alterations for cancer diagnosis and treatment decision. Among the different possible investigations, mRNA analysis provides information on abnormal levels of genes expression. Standard laboratory methods are still not adapted to the isolation and quantitation of low mRNA amounts and new techniques needs to be developed in particular for rare subsets analysis. By reducing the volume involved, time process, and the contamination risks, droplet microfluidics provide numerous advantages to perform analysis down to the single cell level.We report on a droplet microfluidic platform based on the manipulation of magnetic particles that allows the clinical analysis of tumor tissues. In particular, it allows the extraction of mRNA from the total-RNA sample, Reverse Transcription, and cDNA amplification, all in droplets.
- Subjects :
- Magnetic tweezers
Microfluidics
02 engineering and technology
Computational biology
01 natural sciences
Workflow
Reverse Transcription (RT)
Neoplasms
Complementary DNA
Genetics
medicine
Humans
Droplet microfluidics
Typing
Droplet microfluidics PCR
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Molecular Biology
Tumor
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
010401 analytical chemistry
Reproducibility of Results
Cancer
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
medicine.disease
0104 chemical sciences
PCR
Nucleic acid
Magnetic nanoparticles
Reverse transcription (RT)
Biomarkers, Tumor
0210 nano-technology
Biomarkers
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-6732-2
- ISBNs :
- 9781493967322
- Database :
- OpenAIRE
- Journal :
- Microchip Diagnostics, Microchip Diagnostics, pp.113-121, 2017, 978-1-4939-6732-2. ⟨10.1007/978-1-4939-6734-6_9⟩, Methods in Molecular Biology ISBN: 9781493967322
- Accession number :
- edsair.doi.dedup.....399d8b17b8cae1292469766cf7005080